810 Distinct transcriptional profiles in the different phenotypes of neurofibroma from the same neurofibromatosis 1 subject

Neurofibromatosis type1 (NF1) is an autosomal dominant genetic disorder which affects various organs and characterized by systemic neurofibromas (NF). Among NF, cutaneous neurofibroma (cNF) plexiform (pNF) have different clinical manifestations, pNF potentially causes unresectable malignant transformation. To elucidate the distinct tumor microenvironment according to phenotypes of NF thus important for finding clues controlling transformation pNF. Herein, single-cell RNA-sequencing (scRNA-seq) was performed on isolated cNF cells from same NF1 patient. Immunohistochemical analysis also conducted six five specimens subjects. Our results displayed that transcriptional profiles even within subject. Schwann with robust metabolic capacity, fibroblasts cancer-associated fibroblast-like phenotype, M2-like macrophages are enriched in pNF, while CD8 T tissue residency markers effector cytokines endowed cNF. Overall, prone be immunoregulatory proinflammatory. The immunohistochemical analyses were consistent those scRNA-seq. Collectively, our suggest targeting can a candidate preventing